ARG23467

anti-CD200 antibody [OX-2]

anti-CD200 antibody [OX-2] for IHC-Frozen sections,Electron microscopy,Flow cytometry,ICC/IF,Immunoprecipitation and Rat

Overview

Product Description Mouse Monoclonal antibody [OX-2] recognizes CD200
Mouse anti Rat CD200 antibody, clone OX-2 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells.Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation (Ko et al. 2009).This product is routinely tested by flow cytometry on rat thymocytes.
Tested Reactivity Rat
Tested Application EM, FACS, ICC/IF, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone OX-2
Isotype IgG1
Target Name CD200
Antigen Species Rat
Immunogen Rat thymocyte membrane glycoproteins.
Conjugation Un-conjugated
Alternate Names OX-2; OX-2 membrane glycoprotein; MOX1; MOX2; CD antigen CD200; MRC

Application Instructions

Application Suggestion
Tested Application Dilution
EMAssay-dependent
FACS1:50 - 1:100
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD200
Gene Full Name CD200 molecule
Background The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Costimulates T-cell proliferation. May regulate myeloid cell activity in a variety of tissues. [UniProt]
Calculated MW 31 kDa