ARG22523
anti-CD200R antibody [OX-102]
anti-CD200R antibody [OX-102] for IHC-Frozen sections,Flow cytometry,Immunoprecipitation,Western blot and Rat
Overview
| Product Description | Mouse Monoclonal antibody [OX-102] recognizes CD200R This antibody recognizes the rat OX2 (CD200) receptor 1. This antigen is a heavily glycosylated ~60-100 kDa cell surface molecule expressed by cells of the myeloid lineage but not by T or B lymphocytes.Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 has been shown to block the interaction of OX2 receptor 1 with CD200 (Bushell et al. 2008). |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | FACS, IHC-Fr, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | OX-102 |
| Isotype | IgG1 |
| Target Name | CD200R |
| Antigen Species | Rat |
| Immunogen | Membrane fraction of thioglycollate-elicited rat peripheral cells. |
| Conjugation | Un-conjugated |
| Alternate Names | MOX2R; Cell surface glycoprotein CD200 receptor 1; CD200R; Cell surface glycoprotein OX2 receptor 1; OX2R; HCRTR2; CD200 cell surface glycoprotein receptor |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9ES58 Rat Cell surface glycoprotein CD200 receptor 1 |
|---|---|
| Gene Symbol | Cd200r1 |
| Gene Full Name | CD200 receptor 1 |
| Background | This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| Function | Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. [UniProt] |
| Calculated MW | ~ 60 - 100 kDa |
| PTM | The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform. |
