ARG22524

anti-CD200R antibody [OX-102] (low endotoxin)

anti-CD200R antibody [OX-102] (low endotoxin) for IHC-Frozen sections,Flow cytometry,Functional study,Immunoprecipitation,Western blot and Rat

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [OX-102] recognizes CD200R
This antibody recognizes the rat OX2 (CD200) receptor 1. This antigen is a heavily glycosylated ~60-100 kDa cell surface molecule expressed by cells of the myeloid lineage but not by T or B lymphocytes.Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 has been shown to block the interaction of OX2 receptor 1 with CD200 (Bushell et al. 2008).
Tested Reactivity Rat
Tested Application FACS, FuncSt, IHC-Fr, IP, WB
Host Mouse
Clonality Monoclonal
Clone OX-102
Isotype IgG1
Target Name CD200R
Antigen Species Rat
Immunogen Membrane fraction of thioglycollate-elicited rat peripheral cells.
Conjugation Un-conjugated
Alternate Names MOX2R; Cell surface glycoprotein CD200 receptor 1; CD200R; Cell surface glycoprotein OX2 receptor 1; OX2R; HCRTR2; CD200 cell surface glycoprotein receptor

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:10
FuncStAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note Low endotoxin
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64357 Rat CD200R1

Swiss-port # Q9ES58 Rat Cell surface glycoprotein CD200 receptor 1

Gene Symbol Cd200r1
Gene Full Name CD200 receptor 1
Background This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Function Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. [UniProt]
Calculated MW ~ 60 - 100 kDa
PTM The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform.