ARG22996
anti-CD200R antibody [OX-110]
anti-CD200R antibody [OX-110] for Flow cytometry and Mouse
Overview
| Product Description | Rat Monoclonal antibody [OX-110] recognizes CD200R Rat anti Mouse CD200R antibody, clone OX-110 recognizes mouse CD200R, a cell surface glycoprotein (also known as OX2R. Mouse CD200R is a 326 amino acid, ~48 kDa single pass type I transmembrane glycoprotein, expressed primarily by peripheral blood monocytes and neutrophils but also by other leucocytes including T-lymphocytes and mast cells (Wright et al.2003). Studies suggest that CD200-CD200R interaction may be involved in the control of myeloid cellular function (Minas and Liversidge 2006).Rat anti Mouse CD200R antibody, clone OX-110 has been used successfully for the immunohistochemical detection of CD200R expressing cells on acetone fixed cryosections of murine synovial tissue (Simelyte et al. 2008). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | OX-110 |
| Isotype | IgG2a |
| Target Name | CD200R |
| Antigen Species | Mouse |
| Immunogen | Fusion protein mCD200RCD4d3+4 |
| Conjugation | Un-conjugated |
| Alternate Names | MOX2R; Cell surface glycoprotein CD200 receptor 1; CD200R; Cell surface glycoprotein OX2 receptor 1; OX2R; HCRTR2; CD200 cell surface glycoprotein receptor |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9ES57 Mouse Cell surface glycoprotein CD200 receptor 1 |
|---|---|
| Gene Symbol | Cd200r1 |
| Gene Full Name | CD200 receptor 1 |
| Background | This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008] |
| Function | Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of proinflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. [UniProt] |
| Calculated MW | 37 kDa |
| PTM | The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform. |
