ARG22652

anti-CD203a antibody [PM18-7]

anti-CD203a antibody [PM18-7] for IHC-Frozen sections,Flow cytometry,Immunoprecipitation and Pig

Overview

Product Description Mouse Monoclonal antibody [PM18-7] recognizes CD203a
This antibody recognizes Porcine CD203a, originally clustered as SWC9 at the Second International Swine CD Workshop (Dominguez et al. 1998) and later identified as the Porcine homologue of Human ecto-nucleotidepyrophosphatase / phosphodiesterase 1 or ENPP1 (Petersen et al. 2007).
Mouse anti Pig CD203a was originally reported to immunoprecipitate two bands, one of ~;205 kDa and one of ~130 kDa (Dominguez et al. 1998) under both reducing and non-reducing conditions. Subsequent studies suggest that CD203a migrates as a homodimer of ~260 kDa under non-reducing conditions and a 130 kDa monomer under reducing conditions (Petersen et al. 2007) from preparations of Porcine alveolar macrophages.
CD203a is expressed widely in macrophage populations with notably high levels on alveolar macrophages (Petersen et al. 2007, Hwang et al. 2015), it is not expressed on monocyte populations (McCullough et al. 1997, Hwang et al. 2015).
SWC1a, expressed at very much higher levels on monocytes than mature macrophages and CD203a (SWC9), expressed exclusively on mature tissue macrophages have been used as markers of monocyte-macrophage differentiation (Sanchez et al. 1999).
Tested Reactivity Pig
Tested Application FACS, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone PM18-7
Isotype IgG1
Target Name CD203a
Antigen Species Pig
Immunogen Porcine alveolar macrophages.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25 - 1:200
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.