ARG65456

anti-CD205 antibody [HD30]

anti-CD205 antibody [HD30] for Flow cytometry,Western blot,IHC-Frozen sections and Human

Controls and Markers antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [HD30] recognizes CD205
Tested Reactivity Hu
Tested Application FACS, IHC-Fr, WB
Specificity The clone HD30 recognizes CD205, an approx. 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.
Host Mouse
Clonality Monoclonal
Clone HD30
Isotype IgG1
Target Name CD205
Antigen Species Human
Immunogen Recombinant Fc-tagged human CD205
Conjugation Un-conjugated
Alternate Names Lymphocyte antigen 75; DEC-205; DEC205; Ly-75; CD205; CD antigen CD205

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
IHC-FrAssay-dependent
WBAssay-dependent
Application Note WB: Only non-denatured samples.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4065 Human LY75

Swiss-port # O60449 Human Lymphocyte antigen 75

Gene Symbol LY75
Gene Full Name lymphocyte antigen 75
Background CD205, also known as DEC-205, is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.
Function Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes. [UniProt]
Research Area Controls and Markers antibody; Immune System antibody
Calculated MW 198 kDa

Clone References

Generation of anti-human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals.

Park CG et al.
J Immunol Methods.,  (2012)

publication_link

 

hr_line