ARG22874

anti-CD205 antibody [NLDC-145]

anti-CD205 antibody [NLDC-145] for IHC-Frozen sections,Flow cytometry and Mouse

Overview

Product Description Rat Monoclonal antibody [NLDC-145] recognizes CD205
Rat anti Mouse CD205, clone NLDC-145 recognizes DEC-205, a ~205 kDa integral membrane glycoprotein expressed by mouse dendritic cells and thymic epithelial cells. It is absent in bone marrow cells, all blood cells and freshly isolated macrophages from the peritoneal cavity. A subpopulation of the latter becomes positive in mice previously stimulated with thioglycollate. It is also absent on follicular dendritic cells of B cell follicles.The antigen is found on la positive interdigitating cells in T cell areas of all secondary lymphoid organs. It is also present on veiled cells. In non-lymphoid organs, the antigen is only found on Langerhans cells of the skin but cross reactions with la positive thymic epithelial cells and intestinal villus epithelial cells are also observed.DEC-205 is expressed at the cell surface of unfixed cells but can also be found in the cytoplasm of fixed cells.
Tested Reactivity Ms
Tested Application FACS, IHC-Fr
Host Rat
Clonality Monoclonal
Clone NLDC-145
Isotype IgG2a
Target Name CD205
Antigen Species Mouse
Immunogen Mouse lymph node stroma.
Conjugation Un-conjugated
Alternate Names CD205; DEC205; DEC-205

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
IHC-Fr1:10 - 1:25
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Tissue Culture Supernatant.
Buffer Tissue Culture Supernatant, 0.2M Tris/HCl (pH 7.4), 5-10% foetal calf serum and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 17076 Mouse LY75

Swiss-port # Q60767 Mouse Lymphocyte antigen 75

Gene Symbol Ly75
Gene Full Name lymphocyte antigen 75
Function Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B lymphocytes. [UniProt]
Calculated MW 198 kDa