ARG22651

anti-CD21 antibody [CA2.1D6]

anti-CD21 antibody [CA2.1D6] for IHC-Frozen sections,Flow cytometry,Immunoprecipitation and Cat,Horse,Dog

Overview

Product Description Mouse Monoclonal antibody [CA2.1D6] recognizes CD21
This antibody recognizes Canine CD21, also known as Complement receptor type 2. CD21is a cell surface antigen expressed by Canine B lymphocytes.
The antigen recognised may be the Canine homologue of Human CD21, but this has not been fully confirmed.
Mouse anti Canine CD21 antibody , clone CA2.1D6 also recognizes the CD21 antigen in Felids. Expression in cats is analogous to that seen in dogs with strong expression on lymphocytes, in a manner mutually exclusive with expression of CD4 or CD8. Clone CA2.1D6 also immunoprecipitates a 145kDa protein from feline lymphocytes, similar to the protein immunoprecipitated by the antibody from Canine lymphocytes (Dean et al. 1996).
Tested Reactivity Cat, Dog, Hrs
Tested Application FACS, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone CA2.1D6
Isotype IgG1
Target Name CD21
Antigen Species Dog
Conjugation Un-conjugated
Alternate Names Cr2; Complement C3d receptor; C3DR; CD21; CD antigen CD21; Complement receptor type 2; SLEB9; CR; CVID7; Epstein-Barr virus receptor; EBV receptor

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CR2
Gene Full Name complement component (3d/Epstein Barr virus) receptor 2
Background This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Function Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. [UniProt]
Calculated MW 113 kDa