ARG22907

anti-CD21 antibody [LB21]

anti-CD21 antibody [LB21] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Bovine,Cat,Goat,Human,Sheep

Overview

Product Description Mouse Monoclonal antibody [LB21] recognizes CD21
Mouse anti Human CD21 antibody, clone LB21 recognizes the human Complement receptor type 2, also known as CD21 or the Epstein-Barr virus receptor. CD21 is a ~14 kDa cell surface glycoprotein expressed by mature B cells and by follicular dendritic cells. The molecule acts as a receptor for complement components C3d, C3dg and iC3b, as well as for Epstein Barr Virus. It forms part of a large signal transduction complex in association with CD19.
Tested Reactivity Hu, Bov, Cat, Goat, Sheep
Tested Application FACS, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone LB21
Isotype IgG1
Target Name CD21
Antigen Species Human
Immunogen Human IM9 cell line.
Conjugation Un-conjugated
Alternate Names Cr2; Complement C3d receptor; C3DR; CD21; CD antigen CD21; Complement receptor type 2; SLEB9; CR; CVID7; Epstein-Barr virus receptor; EBV receptor

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:10
IHC-FrAssay-dependent
IPAssay-dependent
Application Note IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl human whole blood.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by ion exchange chromatography.
Buffer PBS, 0.09% Sodium azide and 1% BSA
Preservative 0.09% Sodium azide
Stabilizer 1% BSA
Concentration 25 µg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1380 Human CR2

Swiss-port # P20023 Human Complement receptor type 2

Gene Symbol CR2
Gene Full Name complement component (3d/Epstein Barr virus) receptor 2
Background This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Function Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation. [UniProt]
Calculated MW 113 kDa