ARG53795
anti-CD222 / IGF2R antibody [MEM-238] (APC)
anti-CD222 / IGF2R antibody [MEM-238] (APC) for Flow cytometry and Human,Primates
Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody
Overview
| Product Description | APC-conjugated Mouse Monoclonal antibody [MEM-238] recognizes CD222 / IGF2R |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS |
| Specificity | The clone MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-238 |
| Isotype | IgG1 |
| Target Name | CD222 / IGF2R |
| Immunogen | Recombinant Vaccinia virus encoding CD222. |
| Conjugation | APC |
| Alternate Names | CD222; MPR 300; Insulin-like growth factor II receptor; M6P/IGF2R; MPRI; 300 kDa mannose 6-phosphate receptor; IGF-II receptor; CI Man-6-P receptor; M6P/IGF2 receptor; MPR1; CIMPR; Cation-independent mannose-6-phosphate receptor; CD antigen CD222; Insulin-like growth factor 2 receptor; M6P-R; CI-MPR; M6PR |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11717 Human Cation-independent mannose-6-phosphate receptor |
|---|---|
| Gene Symbol | IGF2R |
| Gene Full Name | insulin-like growth factor 2 receptor |
| Background | CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria. |
| Function | Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. [UniProt] |
| Research Area | Controls and Markers antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 274 kDa |
Clone References
Alix serves as an adaptor that allows human parainfluenza virus type 1 to interact with the host cell ESCRT system.
ICC/IF / Human
The C proteins of human parainfluenza virus type 1 block IFN signaling by binding and retaining Stat1 in perinuclear aggregates at the late endosome.
ICC/IF /
Histidine-rich glycoprotein binds heparanase and regulates its enzymatic activity and cell surface interactions.
FACS / Human
The retromer component SNX6 interacts with dynactin p150(Glued) and mediates endosome-to-TGN transport.
ICC/IF / Human
Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression.
Inhib / Human
Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation.
IP / Mouse
Cholesterol dependence of varicella-zoster virion entry into target cells.
ICC/IF / Human
TGF-beta-induced apoptosis in endothelial cells mediated by M6P/IGFII-R and mini-plasminogen.
The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration.
