ARG42332
anti-CD222 / IGF2R antibody [MEM-238] (Biotin)
anti-CD222 / IGF2R antibody [MEM-238] (Biotin) for Flow cytometry,Immunoprecipitation,Western blot and Human,Primates
Overview
| Product Description | Biotin-conjugated Mouse Monoclonal antibody [MEM-238] recognizes CD222 / IGF2R |
|---|---|
| Tested Reactivity | Hu, NHuPrm |
| Tested Application | FACS, IP, WB |
| Specificity | The antibody MEM-238 recognizes an extracellular epitope between amino acids 192-697 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-238 |
| Isotype | IgG1 |
| Target Name | CD222 / IGF2R |
| Antigen Species | Human |
| Immunogen | Recombinant Vaccinia virus encoding CD222. |
| Conjugation | Biotin |
| Alternate Names | CD222; MPR 300; Insulin-like growth factor II receptor; M6P/IGF2R; MPRI; 300 kDa mannose 6-phosphate receptor; IGF-II receptor; CI Man-6-P receptor; M6P/IGF2 receptor; MPR1; CIMPR; Cation-independent mannose-6-phosphate receptor; CD antigen CD222; Insulin-like growth factor 2 receptor; M6P-R; CI-MPR; M6PR |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11717 Human Cation-independent mannose-6-phosphate receptor |
|---|---|
| Gene Symbol | IGF2R |
| Gene Full Name | insulin-like growth factor 2 receptor |
| Background | This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611). [provided by RefSeq, Nov 2015] |
| Function | Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4. [UniProt] |
| Cellular Localization | Lysosome membrane; Single-pass type I membrane protein. Note=Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface. [UniProt] |
| Calculated MW | 274 kDa |
