ARG22550
anti-CD223 / LAG3 antibody [C9B7W]
anti-CD223 / LAG3 antibody [C9B7W] for Flow cytometry,CyTOF®-candidate and Mouse
Overview
| Product Description | Rat Monoclonal antibody [C9B7W] recognizes CD223 / LAG3 This antibody recognizes murine lymphocyte activation gene-3 (LAG-3), a ~70 kDa activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Clone C9B7W recognizes an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II (Workman et al. 2002). |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | CyTOF®-candidate, FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | C9B7W |
| Isotype | IgG1 |
| Target Name | CD223 / LAG3 |
| Antigen Species | Mouse |
| Immunogen | Murine CD223 Ig fusion protein. |
| Conjugation | Un-conjugated |
| Alternate Names | CD antigen CD223; CD223; Protein FDC; Lymphocyte activation gene 3 protein; LAG-3 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q61790 Mouse Lymphocyte activation gene 3 protein |
|---|---|
| Gene Symbol | Lag3 |
| Gene Full Name | lymphocyte-activation gene 3 |
| Background | Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008] |
| Function | Involved in lymphocyte activation. Binds to HLA class-II antigens. [UniProt] |
| Highlight | Related products: CD223 antibodies; Anti-Rat IgG secondary antibodies; Related news: CyTOF-candidate Antibodies |
| Calculated MW | 57 kDa |
