ARG22551

anti-CD223 / LAG3 antibody [C9B7W] (low endotoxin)

anti-CD223 / LAG3 antibody [C9B7W] (low endotoxin) for Flow cytometry,Functional study and Mouse

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [C9B7W] recognizes CD223 / LAG3
This antibody recognizes murine lymphocyte activation gene-3 (LAG-3), a ~70 kDa activation-induced cell surface molecule that is also referred to as CD223. Murine CD223 is expressed on activated CD4 positive and CD8 positive alpha/beta T lymphocytes and a subset of natural killer (NK) cells. CD223 binds to MHC class II molecules with high affinity and is reported to negatively regulate T cell homeostasis and T cell expansion. Clone C9B7W recognizes an epitope within the D2 domain of CD223. Rat anti Mouse CD223 antibody, clone C9B7W is reported to block the in vitro function of murine LAG-3 but does not block binding of LAG-3 to MHC class II (Workman et al. 2002).
Tested Reactivity Ms
Tested Application FACS, FuncSt
Host Rat
Clonality Monoclonal
Clone C9B7W
Isotype IgG1
Target Name CD223 / LAG3
Antigen Species Mouse
Immunogen Murine CD223 Ig fusion protein.
Conjugation Un-conjugated
Alternate Names CD antigen CD223; CD223; Protein FDC; Lymphocyte activation gene 3 protein; LAG-3

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat - 1:5
FuncStAssay-dependent
Application Note FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note Low endotoxin
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16768 Mouse LAG3

Swiss-port # Q61790 Mouse Lymphocyte activation gene 3 protein

Gene Symbol Lag3
Gene Full Name lymphocyte-activation gene 3
Background Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
Function Involved in lymphocyte activation. Binds to HLA class-II antigens. [UniProt]
Calculated MW 57 kDa