ARG23440

anti-CD226 antibody [DX11]

anti-CD226 antibody [DX11] for Flow cytometry,Immunoprecipitation,CyTOF®-candidate and Human

Immune System antibody; T Cell Intercellular Adhesion Molecule antibody

Overview

Product Description Mouse Monoclonal antibody [DX11] recognizes CD226
Mouse anti Human CD226 antibody, clone DX11 recognizes human CD226, a ~65 kDa glycoprotein, also known as DNAM1 (DNAX accessory molecule-1). CD226 is broadly expressed on T-cells, NK cells, platelets, monocytes and a subset of B cells. CD226 is also expressed by a subset of CD3 positive thymocytes.Mouse anti Human CD226 antibody, clone DX11 is reported to inhibit T- and NK cell mediated cytotoxicity against tumor cell targets and to block TNF alpha and IFN gamma secretion by alloantigen-specific T-cells (Kojima et al. 2003).
Tested Reactivity Hu
Tested Application CyTOF®-candidate, FACS, IP
Host Mouse
Clonality Monoclonal
Clone DX11
Isotype IgG1
Target Name CD226
Antigen Species Human
Immunogen Human cytotoxic T lymphocyte clone.
Conjugation Un-conjugated
Alternate Names TLiSA1; CD antigen CD226; PTA1; CD226 antigen; DNAM-1; DNAX accessory molecule 1; DNAM1

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACS1:25 - 1:100
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10666 Human CD226

Swiss-port # Q15762 Human CD226 antigen

Gene Symbol CD226
Gene Full Name CD226 molecule
Background This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Function Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell. [UniProt]
Highlight Related products:
CD226 antibodies; CD226 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
CyTOF-candidate Antibodies
Research Area Immune System antibody; T Cell Intercellular Adhesion Molecule antibody
Calculated MW 39 kDa