ARG62779

anti-CD229 antibody [HLy9.25]

anti-CD229 antibody [HLy9.25] for Flow cytometry,Functional study,ICC/IF,Immunoprecipitation,Western blot and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description

Mouse Monoclonal antibody [HLy9.25] recognizes CD229

Tested Reactivity Hu
Tested Application FACS, FuncSt, ICC/IF, IP, WB
Specificity The clone HLy9.25 (also known as HLy9.1.25) recognizes CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
Host Mouse
Clonality Monoclonal
Clone HLy9.25
Isotype IgG1
Target Name CD229
Immunogen CD299-transfected 300.19 pre-B cell line
Conjugation Un-conjugated
Alternate Names Signaling lymphocytic activation molecule 3; T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; hly9; Lymphocyte antigen 9; mLY9; CD229; SLAMF3; CD antigen CD229; SLAM family member 3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS6 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note Functional studies: Regulation of activation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control FACS: Peripheral blood lymphocytes

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4063 Human LY9

Swiss-port # Q9HBG7 Human T-lymphocyte surface antigen Ly-9

Gene Symbol LY9
Gene Full Name lymphocyte antigen 9
Background CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.
Function May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 72 kDa

Clone References

CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse.

Romero X et al.
J Immunol.,  (2005)

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