ARG53797

anti-CD229 antibody [HLy9.25] (PE)

anti-CD229 antibody [HLy9.25] (PE) for Flow cytometry and Human

Developmental Biology antibody; Immune System antibody

Overview

Product Description

PE-conjugated Mouse Monoclonal antibody [HLy9.25] recognizes CD229

Tested Reactivity Hu
Tested Application FACS
Specificity The clone HLy9.25 (also known as HLy9.1.25) recognizes CD229 / Ly9, a 100-120 kDa cell surface glycoprotein expressed on T and B cells.
Host Mouse
Clonality Monoclonal
Clone HLy9.25
Isotype IgG1
Target Name CD229
Immunogen CD299-transfected 300.19 pre-B cell line
Conjugation PE
Alternate Names Signaling lymphocytic activation molecule 3; T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; hly9; Lymphocyte antigen 9; mLY9; CD229; SLAMF3; CD antigen CD229; SLAM family member 3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4063 Human LY9

Swiss-port # Q9HBG7 Human T-lymphocyte surface antigen Ly-9

Gene Symbol LY9
Gene Full Name lymphocyte antigen 9
Background CD229 (Ly9) is a cell surface receptor of the CD150 family, which includes also e.g. CD48 and CD224. Receptors of this family regulate cytokine production and cytotoxicity of lymphocytes and NK cells. High levels of CD229 are found on T and B cells, where its expression increases during their maturation. It is absent on granulocytes, bone marrow-derived dendritic cells, platelets and erythrocytes. CD229 has been also reported on mouse monocytes and NK cells. CD229 interacts homophilically through its N-terminal domain and localizes to the contact site between T cells and antigen presenting B cells during antigen-dependent immune synapse formation.
Function May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW 72 kDa

Clone References

CD229 (Ly9) lymphocyte cell surface receptor interacts homophilically through its N-terminal domain and relocalizes to the immunological synapse.

Romero X et al.
J Immunol.,  (2005)

publication_link

 

hr_line