ARG53798

anti-CD230 / Prion protein antibody [EM-20]

anti-CD230 / Prion protein antibody [EM-20] for Western blot and Human

Developmental Biology antibody; Neuroscience antibody

Overview

Product Description Mouse Monoclonal antibody [EM-20] recognizes CD230 / Prion protein
Tested Reactivity Hu
Tested Application WB
Specificity The clone EM-20 recognizes human prion protein (PrP). Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.
Host Mouse
Clonality Monoclonal
Clone EM-20
Isotype IgG2a
Target Name CD230 / Prion protein
Antigen Species Human
Immunogen Recombinant human prion protein
Conjugation Un-conjugated
Alternate Names GSS; PrPc; PrP33-35C; PrP27-30; Alternative prion protein; p27-30; CJD; ASCR; CD230; PrP; PRIP; KURU; AltPrP

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.5 µg/ml
Application Note WB: Non-reducing condition are essential.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5621 Human PRNP

Swiss-port # F7VJQ1 Human Alternative prion protein

Gene Symbol PRNP
Gene Full Name prion protein
Background CD230 / Human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD). Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.
Research Area Developmental Biology antibody; Neuroscience antibody
Calculated MW 9 kDa
PTM The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion.
Isoform 2 is sumoylated with SUMO1.