ARG62781

anti-CD235a antibody [HIR2] (FITC)

anti-CD235a antibody [HIR2] (FITC) for Flow cytometry and Human

Cell Biology and Cellular Response antibody

Overview

Product Description

FITC-conjugated Mouse Monoclonal antibody [HIR2] recognizes CD235a

Tested Reactivity Hu
Tested Application FACS
Specificity The clone HIR2 recognizes N-terminal portion of glycophorin A (and weakly of glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.
HLDA VII; WS Code 70299
Host Mouse
Clonality Monoclonal
Clone HIR2
Isotype IgG2b
Target Name CD235a
Antigen Species Human
Immunogen Synthetic peptide (Human, N-terminal)
Conjugation FITC
Alternate Names MN; GPErik; MNS; GPA; GPSAT; PAS-2; MN sialoglycoprotein; CD235a; HGpMiV; CD antigen CD235a; HGpMiXI; Sialoglycoprotein alpha; HGpSta(C); Glycophorin-A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2993 Human GYPA

Swiss-port # P02724 Human Glycophorin-A

Gene Symbol GYPA
Gene Full Name glycophorin A (MNS blood group)
Background CD235a (Glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
Function Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). [UniProt]
Research Area Cell Biology and Cellular Response antibody
Calculated MW 16 kDa
PTM The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).

Clone References

Proteomic analysis of ERK1/2-mediated human sickle red blood cell membrane protein phosphorylation.

WB, IP / Human

Soderblom EJ et al.
Clin Proteomics.,  (2013)

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Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Zhang X et al.
Oncogene.,  (2013)

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A novel fluorescence-based method in forensic science for the detection of blood in situ.

ICC/IF / Human

Thorogate R et al.
Forensic Sci Int Genet.,  (2008)

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[Immunophenotyping study on the blood cells of the patients with paroxysmal nocturnal hemoglobinuria].

Chen G et al.
Zhonghua Xue Ye Xue Za Zhi.,  (1997)

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Flow cytometric analysis of human bone marrow perfusion cultures: erythroid development and relationship with burst-forming units-erythroid.

Rogers CE et al.
Exp Hematol.,  (1996)

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