ARG22529

anti-CD239 antibody [BRIC221]

anti-CD239 antibody [BRIC221] for IHC-Frozen sections,ELISA,Flow cytometry,ICC/IF,Western blot and Human,Pig

Overview

Product Description Mouse Monoclonal antibody [BRIC221] recognizes CD239
This antibody recognizes human CD239, also known as Lutheran antigen or basal cell aghesion molecule.CD239 is a 597 amino acid, ~85 kDa single pass type I membrane glycoprotein. Clone BRIC221 recognizes a monomorphic determinant expresses on both the 85 and 78 kDa Lutheran (Lu) glycoforms (El Nemer et al. 1998). BRIC 221 recognizes an epitope in the fourth extracellular domain of Lu glycoprotein (Parsons et al. 1997). Lutheran glycoprotein is a member of the immunoglobulin superfamily and was designated CD239 (B-CAM) at the 7th leucocyte typing workshop. CD239 is expressed by erythrocytes in the peripheral blood.
Tested Reactivity Hu, Pig
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone BRIC221
Isotype IgG2b
Target Name CD239
Antigen Species Human
Immunogen Human erythrocytes.
Conjugation Un-conjugated
Alternate Names MSK19; Auberger B antigen; F8/G253 antigen; Lutheran antigen; Basal cell adhesion molecule; LU; AU; CD239; B-CAM cell surface glycoprotein; Lutheran blood group glycoprotein; CD antigen CD239

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
WBAssay-dependent
Application Note WB: Non-reducing conditions required.
FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer TRIS buffered glycine and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4059 Human BCAM

Swiss-port # P50895 Human Basal cell adhesion molecule

Gene Symbol BCAM
Gene Full Name basal cell adhesion molecule (Lutheran blood group)
Background This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
Function Laminin alpha-5 receptor. May mediate intracellular signaling. [UniProt]
Calculated MW 67 kDa
PTM Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin.