ARG42264

anti-CD24 antibody [SN3] (APC)

anti-CD24 antibody [SN3] (APC) for Flow cytometry and Human

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [SN3] recognizes CD24
Tested Reactivity Hu
Tested Application FACS
Specificity The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.
Host Mouse
Clonality Monoclonal
Clone SN3
Isotype IgG1
Target Name CD24
Antigen Species Human
Immunogen Glycoproteins purified from Human NALM-1 cell line.
Conjugation APC
Alternate Names Signal transducer CD24; CD antigen CD24; CD24A; Small cell lung carcinoma cluster 4 antigen

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µl / 100 µl of whole blood or 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100133941 Human CD24

Swiss-port # P25063 Human Signal transducer CD24

Gene Symbol CD24
Gene Full Name CD24 molecule
Background This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Function May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells (PubMed:11313396). In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. Plays a role in the control of autoimmunity (By similarity). [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
Calculated MW 8 kDa
PTM Extensively O-glycosylated. [UniProt]