ARG65540
anti-CD24 antibody [SN3]
anti-CD24 antibody [SN3] for Flow cytometry,ICC/IF,IHC-Frozen sections and Human
Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [SN3] recognizes CD24 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr |
| Specificity | The antibody SN3 reacts with CD24, a 3545 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes. HLDA IV; WS Code B 136 HLDA V; WS Code B CD24.7 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SN3 |
| Isotype | IgG1 |
| Target Name | CD24 |
| Antigen Species | Human |
| Immunogen | Glycoproteins purified from human NALM-1 cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | Signal transducer CD24; CD antigen CD24; CD24A; Small cell lung carcinoma cluster 4 antigen |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Immunohistochemistry (frozen sections): tonsil |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD24 |
| Gene Full Name | CD24 molecule |
| Background | CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid. |
| Function | Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. [UniProt] |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 8 kDa |
| PTM | Extensively O-glycosylated. |
Clone References
Enrichment of breast cancer stem-like cells by growth on electrospun polycaprolactone-chitosan nanofiber scaffolds.
FACS /
CD24: a potential new marker in differentiating malignant mesothelioma from pulmonary adenocarcinoma.
IHC / Human
Human mid-trimester amniotic fluid stem cells cultured under embryonic stem cell conditions with valproic acid acquire pluripotent characteristics.
FACS / Human
FUS-CREB3L2/L1-positive sarcomas show a specific gene expression profile with upregulation of CD24 and FOXL1.
ICC/IF / Human
The role of extracellular factors in human metastatic chordoma cell growth in vitro.
WB / Human
