ARG54295
anti-CD24 antibody [SN3] (PE)
anti-CD24 antibody [SN3] (PE) for Flow cytometry and Human
Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody
Overview
| Product Description | PE-conjugated Mouse Monoclonal antibody [SN3] recognizes CD24 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Specificity | The antibody SN3 reacts with CD24, a 3545 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes. HLDA IV; WS Code B 136 HLDA V; WS Code B CD24.7 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SN3 |
| Isotype | IgG1 |
| Target Name | CD24 |
| Antigen Species | Human |
| Immunogen | Glycoproteins purified from human NALM-1 cell line. |
| Conjugation | PE |
| Alternate Names | Signal transducer CD24; CD antigen CD24; CD24A; Small cell lung carcinoma cluster 4 antigen |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CD24 |
| Gene Full Name | CD24 molecule |
| Background | CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid. |
| Function | Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. [UniProt] |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody |
| Calculated MW | 8 kDa |
| PTM | Extensively O-glycosylated. |
Clone References
Enrichment of breast cancer stem-like cells by growth on electrospun polycaprolactone-chitosan nanofiber scaffolds.
FACS /
CD24 and CD44 in salivary gland pleomorphic adenoma and in human salivary gland morphogenesis: differential markers of glandular structure or stem cell indicators?
IHC / Human
Human mid-trimester amniotic fluid stem cells cultured under embryonic stem cell conditions with valproic acid acquire pluripotent characteristics.
FACS / Human
FUS-CREB3L2/L1-positive sarcomas show a specific gene expression profile with upregulation of CD24 and FOXL1.
ICC/IF / Human
Characterization and functional analysis of a slow cycling stem cell-like subpopulation in pancreas adenocarcinoma.
FACS / Human
The role of extracellular factors in human metastatic chordoma cell growth in vitro.
WB / Human
