ARG62407

anti-CD25 antibody [IL2R.1]

anti-CD25 antibody [IL2R.1] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections and Human

Immune System antibody; Pre-B Cell Marker antibody

Overview

Product Description Mouse Monoclonal antibody [IL2R.1] recognizes CD25
Tested Reactivity Hu
Tested Application FACS, IHC-Fr, IHC-P
Specificity Specific to a 55 kDa protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages.
Host Mouse
Clonality Monoclonal
Clone IL2R.1
Isotype IgG1
Target Name CD25
Antigen Species Human
Immunogen BALB/C mice were injected with recombinant human interleukin-2 receptor
Conjugation Un-conjugated
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Application Note FACS: 1/50.
IHC-P: 1/20 - 1/50.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Tonsil

Properties

Form Liquid
Purification Protein A purified
Buffer PBS (pH 7.2), 0.2% BSA and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 0.2% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3559 Human IL2RA

Swiss-port # P01589 Human Interleukin-2 receptor subunit alpha

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
Cellular Localization Membrane.
Research Area Immune System antibody; Pre-B Cell Marker antibody
Calculated MW 31 kDa

Clone References

CD25 is a novel marker of hepatic bile canaliculus.

IHC / Human

Lu ZH et al.
Int J Surg Pathol.,  (2012)

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FOXP3 T regulatory cells in lesions of oral lichen planus correlated with disease activity.

IHC / Human

Tao XA et al.
Oral Dis.,  (2010)

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Regulatory T cells and liver pathology in a murine graft versus host response model.

IHC / Mouse

Miyazaki T et al.
Hepatol Res.,  (2009)

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Pathway analysis of coronary atherosclerosis.

IHC-Fr / Human

King JY et al.
Physiol Genomics.,  (2005)

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Focal complex formation in adult cardiomyocytes is accompanied by the activation of beta3 integrin and c-Src.

WB / Human

Willey CD et al.
J Mol Cell Cardiol.,  (2003)

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