ARG22829

anti-CD25 antibody [K231.3B2]

anti-CD25 antibody [K231.3B2] for IHC-Frozen sections,Flow cytometry and Pig

Immune System antibody; Pre-B Cell Marker antibody

Overview

Product Description Mouse Monoclonal antibody [K231.3B2] recognizes CD25
Mouse anti Pig CD25, clone K231.3B2 recognizes porcine CD25, the alpha chain of the interleukin 2 receptor (IL-2Rα), also known as the low affinity Interleukin 2 receptor. The IL-2 receptor exists in three forms, the high affinity heterodimer, the intermediate affinity β monomer and the low affinity α monomer configurations. Clone K231.3B2 was clustered as CD25 at the First International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Lunney et al. 1994).Mouse anti pig CD25, clone K231.3B2 immunoprecipitates a protein of ~65-70 kDa from activated lymphocyte preparations (Bailey et al. 1992). CD25 is a 270 amino acid single pass type I transmembrane glycoprotein containing 2 Sushi domains. Low expression of CD25 is seen on resting peripheral blood mononuclear cells, rapidly up-regulated following stimulation by concanavalin A and phorbol myristate acetate, indicative of its role as an activation antigen (Bullido et al. 1999).
Tested Reactivity Pig
Tested Application FACS, IHC-Fr
Host Mouse
Clonality Monoclonal
Clone K231.3B2
Isotype IgG1
Target Name CD25
Antigen Species Pig
Immunogen Con A activated Porcine peripheral blood lymphocytes.
Conjugation Un-conjugated
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25 - 1:200
IHC-FrAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 396814 Pig IL2RA

Swiss-port # O02733 Pig Interleukin-2 receptor subunit alpha

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
Research Area Immune System antibody; Pre-B Cell Marker antibody
Calculated MW 31 kDa