ARG22714

anti-CD25 antibody [KEI-alpha1]

anti-CD25 antibody [KEI-alpha1] for IHC-Frozen sections,Flow cytometry,Immunoprecipitation and Rabbit

Immune System antibody; Pre-B Cell Marker antibody

Overview

Product Description Mouse Monoclonal antibody [KEI-alpha1] recognizes CD25
This antibody recognizes Rabbit CD5, a 55kDa cell surface antigen, otherwise known as the interleukin-2 (IL-2) receptor alpha chain. In Rabbits, CD25 is expressed on activated T lymphocytes.
Mouse anti Rabbit CD25 antibody, clone KEI-alpha1 is reported to inhibit the binding of IL-2 to its receptor (Kotani et al. 1993).
Tested Reactivity Rb
Species Does Not React With Hu
Tested Application FACS, IHC-Fr, IP
Host Mouse
Clonality Monoclonal
Clone KEI-alpha1
Isotype IgG2b
Target Name CD25
Antigen Species Rabbit
Immunogen Cell membrane preparation of HTLV-1 transformed rabbit T cell line.
Conjugation Un-conjugated
Alternate Names IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100 - 1:200
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol IL2RA
Gene Full Name interleukin 2 receptor, alpha
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009]
Function Receptor for interleukin-2. [UniProt]
Research Area Immune System antibody; Pre-B Cell Marker antibody
Calculated MW 31 kDa