ARG23024
anti-CD25 antibody [OX-39] (PE)
anti-CD25 antibody [OX-39] (PE) for Flow cytometry and Rat
Immune System antibody; Pre-B Cell Marker antibody
Overview
Product Description | PE-conjugated Mouse Monoclonal antibody [OX-39] recognizes CD25 Mouse anti Rat CD25 antibody, clone OX-39 recognizes the alpha chain of rat CD25, otherwise known as IL-2 receptor alpha, a ~55 kDa type I membrane glycoprotein, expressed by activated T cells but not resting lymphocytes. CD25 is also expressed by dendritic cells found in the thymus medulla.Mouse anti Rat CD25 antibody, clone OX-39 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).Mouse anti Rat CD25 antibody, clone OX-39 has been shown to weakly inhibit the binding of IL-2 to Con-A stimulated spleen blasts (Paterson et al. 1987). |
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Tested Reactivity | Rat |
Tested Application | FACS |
Host | Mouse |
Clonality | Monoclonal |
Clone | OX-39 |
Isotype | IgG1 |
Target Name | CD25 |
Antigen Species | Rat |
Immunogen | Stimulated Rat T cells |
Conjugation | PE |
Alternate Names | IL-2-RA; IL-2 receptor subunit alpha; CD25; TCGFR; TAC antigen; IL2R; CD antigen CD25; Interleukin-2 receptor subunit alpha; IL-2R subunit alpha; p55; IL2-RA; IDDM10 |
Application Instructions
Application Suggestion |
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Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Purification with Protein G. |
Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose |
Preservative | 0.09% Sodium azide |
Stabilizer | 1% BSA and 5% Sucrose |
Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # P26897 Rat Interleukin-2 receptor subunit alpha |
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Gene Symbol | Il2ra |
Gene Full Name | interleukin 2 receptor, alpha |
Background | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.[provided by RefSeq, Nov 2009] |
Function | Receptor for interleukin-2. [UniProt] |
Research Area | Immune System antibody; Pre-B Cell Marker antibody |
Calculated MW | 31 kDa |