ARG23640
anti-CD252 / OX40L antibody [ATM-2]
anti-CD252 / OX40L antibody [ATM-2] for Flow cytometry and Rat
Overview
| Product Description | Mouse Monoclonal antibody [ATM-2] recognizes CD252 / OX40L. This product specifically recognizes rat CD252, otherwise known as OX40L or CD134L, a ~22 kDa type II transmembrane glycoprotein and member of the TNF superfamily, expressed by antigen-presenting cells including activated dendritic cells and B cells, and also by vascular endothelium. The interaction of CD252 with its receptor OX40/CD134, acts as a co-stimulator of T cell activation, proliferation and both Th1 and Th2 cytokine production, and is also important in activated B cell proliferation and secretion of immunoglobulins (Igs). CD252/OX40 interaction has been implicated in HTLV-1 associated diseases including myelopathy and adult T cell leukaemia, and also with the autoimmune diseases rheumatoid arthritis and inflammatory bowel disease. Mouse anti Rat CD252 antibody, clone ATM-2 has been shown to block the co-stimulatory activity of CD252 on rat T cell proliferation. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ATM-2 |
| Isotype | IgG1 |
| Target Name | CD252 / OX40L |
| Antigen Species | Rat |
| Immunogen | ACI-S1 Rat T-cell lymphoma cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | OX4OL; GP34; Glycoprotein Gp34; TXGP1; OX-40L; CD252; Tumor necrosis factor ligand superfamily member 4; OX40 ligand; OX40L; CD antigen CD252; TAX transcriptionally-activated glycoprotein 1; CD134L |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9Z2P3 Rat Tumor necrosis factor ligand superfamily member 4 |
|---|---|
| Gene Symbol | TNFSF4 |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 4 |
| Background | This gene encodes a cytokine of the tumor necrosis factor (TNF) ligand family. The encoded protein functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Function | Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. [UniProt] |
| Calculated MW | 21 kDa |
