ARG42383
anti-CD255 / TWEAK antibody [CARL-1]
anti-CD255 / TWEAK antibody [CARL-1] for Flow cytometry,Immunohistochemistry and Human
Overview
| Product Description | Mouse Monoclonal antibody [CARL-1] recognizes CD255 / TWEAK |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC |
| Specificity | The mouse monoclonal antibody CARL-1 recognizes an extracellular epitope of CD255 / TWEAK, a type II transmembrane protein of the TNF superfamily able to induce apoptosis weakly in many cell types. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | CARL-1 |
| Isotype | IgG3, kappa |
| Target Name | CD255 / TWEAK |
| Antigen Species | Human |
| Immunogen | Human CD255-transfected 2PK-3 cells. |
| Conjugation | Un-conjugated |
| Alternate Names | APO3L; DR3LG; TWEAK; TNLG4A |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 15 mM Sodium azide. |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43508 Human Tumor necrosis factor ligand superfamily member 12 |
|---|---|
| Gene Symbol | TNFSF12 |
| Gene Full Name | TNF superfamily member 12 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010] |
| Function | Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. [UniProt] |
| Cellular Localization | Plasma membrane; Extracellular region or secreted. [UniProt] |
