ARG42384

anti-CD255 / TWEAK antibody [CARL-1] (low endotoxin)

anti-CD255 / TWEAK antibody [CARL-1] (low endotoxin) for Flow cytometry,Immunohistochemistry,Functional study and Human

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [CARL-1] recognizes CD255 / TWEAK
Tested Reactivity Hu
Tested Application FACS, FuncSt, IHC
Specificity The mouse monoclonal antibody CARL-1 recognizes an extracellular epitope of CD255 / TWEAK, a type II transmembrane protein of the TNF superfamily able to induce apoptosis weakly in many cell types.
Host Mouse
Clonality Monoclonal
Clone CARL-1
Isotype IgG3, kappa
Target Name CD255 / TWEAK
Antigen Species Human
Immunogen Human CD255-transfected 2PK-3 cells.
Conjugation Un-conjugated
Alternate Names APO3L; DR3LG; TWEAK; TNLG4A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
FuncStAssay-dependent
IHCAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein.
Buffer PBS
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8742 Human TNFSF12

Swiss-port # O43508 Human Tumor necrosis factor ligand superfamily member 12

Gene Symbol TNFSF12
Gene Full Name TNF superfamily member 12
Background The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]
Function Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. [UniProt]
Cellular Localization Plasma membrane; Extracellular region or secreted. [UniProt]