ARG42457

anti-CD26 / DPP4 antibody [H194-112]

anti-CD26 / DPP4 antibody [H194-112] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Mouse

Overview

Product Description Rat Monoclonal antibody [H194-112] recognizes CD26 / DPP4
Tested Reactivity Ms
Tested Application FACS, IHC-Fr, IP
Specificity The rat monoclonal antibody H194-112 recognizes an extracellular epitope of CD26, a 110 kDa type II membrane glycoprotein, which is a peptidase expressed on mature thymocytes, T cells (especially activated), B cells, NK cells and macrophages.
Host Rat
Clonality Monoclonal
Clone H194-112
Isotype IgG2a, kappa
Target Name CD26 / DPP4
Antigen Species Mouse
Immunogen BALB/c thymocytes.
Conjugation Un-conjugated
Alternate Names T-cell activation antigen CD26; ADCP2; ADCP-2; DPP IV; Adenosine deaminase complexing protein 2; CD26; EC 3.4.14.5; ADABP; Dipeptidyl peptidase IV soluble form; Dipeptidyl peptidase IV; Dipeptidyl peptidase 4; Dipeptidyl peptidase IV membrane form; TP103; DPPIV; CD antigen CD26

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
IHC-FrAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 15 mM Sodium azide.
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13482 Mouse DPP4

Swiss-port # P28843 Mouse Dipeptidyl peptidase 4

Gene Symbol DPP4
Gene Full Name dipeptidyl-peptidase 4
Background The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]
Function Cell surface glycoprotein receptor involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. Its binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Its interaction with ADA also regulates lymphocyte-epithelial cell adhesion. In association with FAP is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May be involved in the promotion of lymphatic endothelial cells adhesion, migration and tube formation. When overexpressed, enhanced cell proliferation, a process inhibited by GPC3. Acts also as a serine exopeptidase with a dipeptidyl peptidase activity that regulates various physiological processes by cleaving peptides in the circulation, including many chemokines, mitogenic growth factors, neuropeptides and peptide hormones. Removes N-terminal dipeptides sequentially from polypeptides having unsubstituted N-termini provided that the penultimate residue is proline. [UniProt]
Cellular Localization Dipeptidyl peptidase 4 soluble form: Secreted. Cell membrane. Apical cell membrane. Cell projection, invadopodium membrane. Cell projection, lamellipodium membrane. Cell junction. Membrane raft. [UniProt]
Calculated MW 88 kDa
PTM The soluble form (Dipeptidyl peptidase 4 soluble form also named SDPP) derives from the membrane form (Dipeptidyl peptidase 4 membrane form also named MDPP) by proteolytic processing.

N- and O-Glycosylated.

Phosphorylated. Mannose 6-phosphate residues in the carbohydrate moiety are necessary for interaction with IGF2R in activated T-cells. Mannose 6-phosphorylation is induced during T-cell activation. [UniProt]