ARG65436

anti-CD261 / TRAIL R1 antibody [DR-4-02]

anti-CD261 / TRAIL R1 antibody [DR-4-02] for Flow cytometry,ICC/IF,Immunoprecipitation,Functional study and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [DR-4-02] recognizes CD261 / TRAIL R1
Tested Reactivity Hu
Tested Application FACS, FuncSt, ICC/IF, IP
Specificity The clone DR-4-02 recognizes TRAIL-R1 (DR4), a human death receptor 4 (468 amino acids) expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.
Host Mouse
Clonality Monoclonal
Clone DR-4-02
Isotype IgG1
Target Name CD261 / TRAIL R1
Antigen Species Human
Immunogen Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
Conjugation Un-conjugated
Alternate Names TNF-related apoptosis-inducing ligand receptor 1; CD antigen CD261; TRAILR-1; DR4; Tumor necrosis factor receptor superfamily member 10A; CD261; Death receptor 4; APO2; TRAIL receptor 1; TRAIL-R1; TRAILR1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
FuncSt2 - 3 µg/ml
ICC/IFAssay-dependent
IPAssay-dependent
Application Note Functional studies: Soluble clone DR-4-02 blocks apoptosis triggered by a ligand (TRAIL). Plastic-immobilized (cross-linked) DR-4-02 induces apoptosis in sensitive cells. Recommended dilution of antibody: 2-3 μg/ml in cultivation medium Final concentration of TRAIL: 20-200 ng/ml. Application note: It is recommended to add the antibody 15 min before addition of TRAIL.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8797 Human TNFRSF10A

Swiss-port # O00220 Human Tumor necrosis factor receptor superfamily member 10A

Gene Symbol TNFRSF10A
Gene Full Name tumor necrosis factor receptor superfamily, member 10a
Background TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues.
Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).
Function Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody
Calculated MW 50 kDa

Clone References

Systems analysis of apoptosis protein expression allows the case-specific prediction of cell death responsiveness of melanoma cells.

Passante E et al.
Cell Death Differ.,  (2013)

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Evaluating the effect of therapeutic stem cells on TRAIL resistant and sensitive medulloblastomas.

FACS / Human

Nesterenko I et al.
PLoS One.,  (2012)

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Cisplatin and a potent platinum(IV) complex-mediated enhancement of TRAIL-induced cancer cells killing is associated with modulation of upstream events in the extrinsic apoptotic pathway.

ICC/IF / Human

Vondálová Blanárová O et al.
Carcinogenesis.,  (2011)

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Scatter factor protects tumor cells against apoptosis caused by TRAIL.

IP / 

Fan S et al.
Anticancer Drugs.,  (2010)

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