ARG65607

anti-CD261 / TRAIL R1 antibody [DR-4-02] (low endotoxin)

anti-CD261 / TRAIL R1 antibody [DR-4-02] (low endotoxin) for Flow cytometry,Immunoprecipitation,ICC/IF,Functional study and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody

Overview

Product Description Low endotoxin Mouse Monoclonal antibody [DR-4-02] recognizes CD261 / TRAIL R1
Tested Reactivity Hu
Tested Application FACS, FuncSt, ICC/IF, IP
Specificity This antibody recognizes TRAIL-R1 (DR4), a human death receptor 4 (468 amino acids) expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.
Host Mouse
Clonality Monoclonal
Clone DR-4-02
Isotype IgG1
Target Name CD261 / TRAIL R1
Antigen Species Human
Immunogen Fusion protein containing Human TRAIL-R1 (extracellular part) and Human IgG1 heavy chain (constant part).
Conjugation Un-conjugated
Alternate Names TNF-related apoptosis-inducing ligand receptor 1; CD antigen CD261; TRAILR-1; DR4; Tumor necrosis factor receptor superfamily member 10A; CD261; Death receptor 4; APO2; TRAIL receptor 1; TRAIL-R1; TRAILR1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS2 - 4 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IPAssay-dependent
Application Note Functional Application:
Soluble antibody DR-4-02 blocks apoptosis triggered by a ligand (TRAIL).
Plastic-immobilized (cross-linked) DR-4-02 antibody induces apoptosis in sensitive cells.
Recommended dilution of antibody: 2-3 μg/ml in cultivation medium
Final concentration of TRAIL: 20-200 ng/ml
Application note: It is recommended to add the antibody 15 min before addition of TRAIL.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 8797 Human TNFRSF10A

Swiss-port # O00220 Human Tumor necrosis factor receptor superfamily member 10A

Gene Symbol TNFRSF10A
Gene Full Name tumor necrosis factor receptor superfamily, member 10a
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [provided by RefSeq, Jul 2008]
Function Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody
Calculated MW 50 kDa

Clone References

Systems analysis of apoptosis protein expression allows the case-specific prediction of cell death responsiveness of melanoma cells.

Passante E et al.
Cell Death Differ.,  (2013)

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Evaluating the effect of therapeutic stem cells on TRAIL resistant and sensitive medulloblastomas.

FACS / Human

Nesterenko I et al.
PLoS One.,  (2012)

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Cisplatin and a potent platinum(IV) complex-mediated enhancement of TRAIL-induced cancer cells killing is associated with modulation of upstream events in the extrinsic apoptotic pathway.

ICC/IF / Human

Vondálová Blanárová O et al.
Carcinogenesis.,  (2011)

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Scatter factor protects tumor cells against apoptosis caused by TRAIL.

IP / 

Fan S et al.
Anticancer Drugs.,  (2010)

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Matrix protein CCN1 is critical for prostate carcinoma cell proliferation and TRAIL-induced apoptosis.

Franzen CA et al.
Mol Cancer Res.,  (2009)

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