ARG54411

anti-CD262 / TRAIL R2 antibody

anti-CD262 / TRAIL R2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CD262 / TRAIL R2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Specificity This antibody recognizes full-length human and mouse DR5 (57kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD262 / TRAIL R2
Antigen Species Human
Immunogen Peptide corresponding to aa 388-407 of human DR5 precursor (accession no. AF012535).
Conjugation Un-conjugated
Alternate Names TRICK2A; TRICK2B; KILLER; TRAILR2; TNF-related apoptosis-inducing ligand receptor 2; DR5; CD antigen CD262; TRICK2; CD262; KILLER/DR5; Tumor necrosis factor receptor superfamily member 10B; Death receptor 5; TRAIL-R2; TRAIL receptor 2; TRICKB; ZTNFR9

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5 µg/ml
IHC-PAssay-dependent
WB1:250 - 1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa and K562

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 21933 Mouse TNFRSF10B

GeneID: 8795 Human TNFRSF10B

Swiss-port # O14763 Human Tumor necrosis factor receptor superfamily member 10B

Swiss-port # Q9QZM4 Mouse Tumor necrosis factor receptor superfamily member 10B

Gene Symbol TNFRSF10B
Gene Full Name tumor necrosis factor receptor superfamily, member 10b
Background Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain-containing receptors. TRAIL/Apo2L is a new member of the TNF family. A novel death domain-containing receptor for TRAIL was recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several independent laboratories. Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL-induced cell death. Overexpression of DR5 induces apoptosis and activates NF- B.
Function Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Signaling Transduction antibody
Calculated MW 48 kDa