ARG65467

anti-CD266 / TWEAK R antibody [ITEM-4] (low endotoxin)

anti-CD266 / TWEAK R antibody [ITEM-4] (low endotoxin) for Flow cytometry,Functional study,IHC-Frozen sections,Western blot and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [ITEM-4] recognizes CD266 / TWEAK R
Tested Reactivity Hu, Ms
Tested Application FACS, FuncSt, IHC-Fr, WB
Specificity The clone ITEM-4 recognizes CD266 / TWEAK R, a TNFR superfamily receptor for CD255 / TWEAK, a TNF-like weak inducer of apoptosis.
Host Mouse
Clonality Monoclonal
Clone ITEM-4
Isotype IgG2b
Target Name CD266 / TWEAK R
Antigen Species Human
Immunogen human CD266-transfected P815 cells
Conjugation Un-conjugated
Alternate Names Tumor necrosis factor receptor superfamily member 12A; FGF-inducible 14; CD266; Fibroblast growth factor-inducible immediate-early response protein 14; TWEAKR; TweakR; CD antigen CD266; Tweak-receptor; FN14

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
FuncStAssay-dependent
IHC-FrAssay-dependent
WBAssay-dependent
Application Note Functional studies: Blocking.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/��g of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27279 Mouse TNFRSF12A

GeneID: 51330 Human TNFRSF12A

Swiss-port # Q9CR75 Mouse Tumor necrosis factor receptor superfamily member 12A

Swiss-port # Q9NP84 Human Tumor necrosis factor receptor superfamily member 12A

Gene Symbol TNFRSF12A
Gene Full Name tumor necrosis factor receptor superfamily, member 12A
Background CD266 / TWEAK R (TNFRSF12A), also known as FN14 (fibroblast growth factor-inducible 14) is a receptor for CD255 / TWEAK, the TNF-like weak inducer of apoptosis. CD266 is expressed on endothelial cells, as well as on some cancer tissues, and plays a role in CD255-induced endothelial cell migration, proliferation, and angiogenesis. The CD255-CD266 interaction, or antibody-mediated triggering of CD266 is also able to induce apoptosis and necrosis in CD266-positive cells (including tumor cells), which might have therapeutic potential.
Function Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 14 kDa

Clone References

TWEAK mediates signal transduction and differentiation of RAW264.7 cells in the absence of Fn14/TweakR. Evidence for a second TWEAK receptor.

Polek TC et al.
J Biol Chem.,  (2003)

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