ARG41435

anti-CD271 / NGFR p75 antibody

anti-CD271 / NGFR p75 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CD271 / NGFR p75
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD271 / NGFR p75
Antigen Species Human
Immunogen Synthetic peptide of Human CD271 / NGFR p75.
Conjugation Un-conjugated
Alternate Names Gp80-LNGFR; CD271; CD antigen CD271; p75 ICD; Tumor necrosis factor receptor superfamily member 16; p75NTR; NGF receptor; TNFRSF16; p75(NTR); Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50
WB1:10000 - 1:20000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control C6
Observed Size ~ 73 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24596 Rat NGFR

GeneID: 4804 Human NGFR

Swiss-port # P07174 Rat Tumor necrosis factor receptor superfamily member 16

Swiss-port # P08138 Human Tumor necrosis factor receptor superfamily member 16

Gene Symbol NGFR
Gene Full Name nerve growth factor receptor
Background Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]
Function Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 45 kDa
PTM N- and O-glycosylated.

O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.

Phosphorylated on serine residues. [UniProt]