ARG65518
anti-CD271 / NGFR p75 antibody [NGFR5]
anti-CD271 / NGFR p75 antibody [NGFR5] for CyTOF®-candidate,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Cat,Ferret,Primates,Rabbit
Developmental Biology antibody; Neuroscience antibody
Overview
| Product Description | Mouse Monoclonal antibody [NGFR5] recognizes CD271 / NGFR p75 |
|---|---|
| Tested Reactivity | Hu, Cat, Frt, NHuPrm, Rb |
| Species Does Not React With | Ms, Rat |
| Tested Application | CyTOF®-candidate, FACS, ICC/IF, IHC-P, IP, WB |
| Specificity | The mouse monoclonal antibody NGFR5 (originally C34C) recognizes CD271 / NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily. The epitope is localized within amino acids 1 - 160. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NGFR5 |
| Isotype | IgG1 |
| Target Name | CD271 / NGFR p75 |
| Antigen Species | Human |
| Immunogen | Purified CD271 protein isolated from human melanoma cell line A875 |
| Conjugation | Un-conjugated |
| Alternate Names | Gp80-LNGFR; CD271; CD antigen CD271; p75 ICD; Tumor necrosis factor receptor superfamily member 16; p75NTR; NGF receptor; TNFRSF16; p75(NTR); Low affinity neurotrophin receptor p75NTR; Low-affinity nerve growth factor receptor |
Application Instructions
| Application Suggestion |
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| Application Note | Immunohistochemistry (paraffin sections): Positive tissue: melanoma Application note: heat-mediated antigen retrieval * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P08138 Human Tumor necrosis factor receptor superfamily member 16 |
|---|---|
| Gene Symbol | NGFR |
| Gene Full Name | nerve growth factor receptor |
| Background | CD271 / NGFR, also known as p75NGFR or p75NTR, is a 75 kDa low affinity receptor for the NGF (nerve growth factor), BDNF (brain-derived growth factor), and other neurotrophins, such as NT3 and NT4/5. Unlike other members of the tumor necrosis factor receptor superfamily of transmembrane proteins, CD271 has unique intracellular domain structure (lacks catalytic activity) and downstream signaling partners. Triggered by its ligands CD271 affects growth, differentiation, migration and death of the nervous system cells. |
| Function | Plays a role in the regulation of the translocation of GLUT4 to the cell surface in adipocytes and skeletal muscle cells in response to insulin, probably by regulating RAB31 activity, and thereby contributes to the regulation of insulin-dependent glucose uptake (By similarity). Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. Necessary for the circadian oscillation of the clock genes ARNTL/BMAL1, PER1, PER2 and NR1D1 in the suprachiasmatic nucleus (SCN) of the brain and in liver and of the genes involved in glucose and lipid metabolism in the liver. [UniProt] |
| Research Area | Developmental Biology antibody; Neuroscience antibody |
| Calculated MW | 45 kDa |
| PTM | N- and O-glycosylated. O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. Phosphorylated on serine residues. |
Clone References
Induced periosteum a complex cellular scaffold for the treatment of large bone defects.
IHC-P, FACS / Human
Pathology of experimental aerosol Zaire ebolavirus infection in rhesus macaques.
The dimensions and characteristics of the subepidermal nerve plexus in human skin--terminal Schwann cells constitute a substantial cell population within the superficial dermis.
ICC/IF / Human
Rac1b increases with progressive tau pathology within cholinergic nucleus basalis neurons in Alzheimer's disease.
IHC / Human
Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271.
IHC-P / Human
Growth of benign and malignant schwannoma xenografts in severe combined immunodeficiency mice.
IHC / Mouse
Modulation of Fas-induced apoptosis by p75 neurotrophin receptor in a human neuroblastoma cell line.
WB / Human
Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells.
FACS / Human
