ARG43316

anti-CD272 / BTLA antibody

anti-CD272 / BTLA antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,Flow cytometry,Immunoprecipitation and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes CD272 / BTLA
Tested Reactivity Hu, Ms
Tested Application FACS, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD272 / BTLA
Antigen Species Mouse
Immunogen Recombinant protein of Mouse CD272 / BTLA.
Conjugation Un-conjugated
Alternate Names CD antigen CD272; BTLA1; B- and T-lymphocyte-associated protein; B- and T-lymphocyte attenuator; CD272

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
IHC-P1:20
IP1:20
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562
Observed Size ~ 72 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer 50 nM Tris-Glycine (pH 7.4), 0.15M NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 151888 Human BTLA

GeneID: 208154 Mouse BTLA

Swiss-port # Q7TSA3 Mouse B- and T-lymphocyte attenuator

Swiss-port # Q7Z6A9 Human B- and T-lymphocyte attenuator

Gene Symbol BTLA
Gene Full Name B and T lymphocyte associated
Background This gene encodes a member of the immunoglobulin superfamily. The encoded protein contains a single immunoglobulin (Ig) domain and is a receptor that relays inhibitory signals to suppress the immune response. Alternative splicing results in multiple transcript variants. Polymorphisms in this gene have been associated with an increased risk of rheumatoid arthritis. [provided by RefSeq, Aug 2011]
Function Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006, PubMed:15568026, PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044). [UniProt]
Cellular Localization Membrane; Single-pass type I membrane protein. [UniProt]
Calculated MW 33 kDa
PTM Phosphorylated on Tyr residues by TNFRSF14 and by antigen receptors cross-linking, both inducing association with PTPN6 and PTPN11.

N-glycosylated. [UniProt]