ARG22561

anti-CD273 / PD-L2 antibody [TY25]

anti-CD273 / PD-L2 antibody [TY25] for Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Mouse

Overview

Product Description Rat Monoclonal antibody [TY25] recognizes CD273 / PD-L2
This antibody recognizes mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). Studies suggest that both CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production.Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004). Removal of sodium azide is recommended prior to use in functional assays, the use of EQU003 is recommended for this purpose.
Tested Reactivity Ms
Tested Application FACS, IHC-Fr, IP
Host Rat
Clonality Monoclonal
Clone TY25
Isotype IgG2a
Target Name CD273 / PD-L2
Antigen Species Mouse
Immunogen B7-DC transfected RAW264.7 cells.
Conjugation Un-conjugated
Alternate Names B7DC; bA574F11.2; PDL2; CD antigen CD273; PD-L2; Programmed cell death 1 ligand 2; CD273; Btdc; PDCD1L2; Programmed death ligand 2; Butyrophilin B7-DC; B7-DC; PD-1 ligand 2; PDCD1 ligand 2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100 - 1:200
IHC-FrAssay-dependent
IPAssay-dependent
Application Note FACS: Use 10ul of the suggested working dilution to label 10^6 cells in 100ul.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 58205 Mouse PDCD1LG2

Swiss-port # Q9WUL5 Mouse Programmed cell death 1 ligand 2

Gene Symbol Pdcd1lg2
Gene Full Name programmed cell death 1 ligand 2
Function Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production (By similarity). [UniProt]
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Calculated MW 31 kDa