ARG11040

anti-CD274 / PD-L1 antibody [15G3]

anti-CD274 / PD-L1 antibody [15G3] for Sandwich ELISA,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [15G3] recognizes CD274 / PD-L1
Tested Reactivity Hu
Tested Application WB, sELISA
Specificity Reactive with human programmed death ligand 1 (PD-L1).
Host Mouse
Clonality Monoclonal
Clone 15G3
Isotype IgG1, kappa
Target Name CD274 / PD-L1
Antigen Species Human
Immunogen Human cell expressed recombinant PD-L1 (Phe19-Arg238) with poly-histidine tag at C-terminus.
Conjugation Un-conjugated
Alternate Names Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1

Application Instructions

Application Suggestion
Tested Application Dilution
WBAssay-dependent
sELISAAssay-dependent
Application Note sELISA: Recommended pairs (capture - detection): ARG11040 anti-CD274 / PD-L1 antibody [15G3] - ARG11039 anti-CD274 / PD-L1 antibody [12B4]
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.0)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29126 Human CD274

Swiss-port # Q9NZQ7 Human Programmed cell death 1 ligand 1

Gene Symbol CD274
Gene Full Name CD274 molecule
Background CD274 / PD-L1 is an immune inhibitory receptor ligand. It is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Function CD274 / PD-L1 plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function. The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy. [UniProt]
Cellular Localization Cell membrane and Endomembrane system.
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Calculated MW 33 kDa