ARG23619

anti-CD279 / PD-1 antibody [RMP1-30]

anti-CD279 / PD-1 antibody [RMP1-30] for Flow cytometry,CyTOF®-candidate and Mouse

Overview

Product Description Rat Monoclonal antibody [RMP1-30] recognizes CD279 / PD-1.
This product recognizes mouse CD279, a ~55 kDa cell surface protein, a member of the CD28/CTLA-4 family, otherwise known as Programmed Death-1 (PD-1). CD279 is expressed predominantly on activated T- and B- lymphocytes and on a subset of thymocytes. Studies suggest that CD279, an immunoinhibitory receptor, plays a critical role in peripheral tolerance induction and prevention of autoimmune disease. Two members of the B7 family, B7-H1 (PD-L1) and B7-DC (PD-L2), have been identified as the ligands for CD279. Rat anti Mouse CD279 antibody, clone RMP1-30 does not block the binding of either B7-H1-Ig or B7-DC-Ig fusion proteins to PD-1 transfected BHK cells.
Tested Reactivity Ms
Tested Application CyTOF®-candidate, FACS
Host Rat
Clonality Monoclonal
Clone RMP1-30
Isotype IgG2b
Target Name CD279 / PD-1
Antigen Species Mouse
Immunogen PD-1 transferred BHK cells.
Conjugation Un-conjugated
Alternate Names hPD-l; CD279; PD-1; Protein PD-1; CD antigen CD279; PD1; hSLE1; SLEB2; Programmed cell death protein 1; hPD-1

Application Instructions

Application Suggestion
Tested Application Dilution
CyTOF®-candidateAssay-dependent
FACSNeat
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18566 Mouse PDCD1

Swiss-port # Q02242 Mouse Programmed cell death protein 1

Gene Symbol PDCD1
Gene Full Name programmed cell death 1
Background CD279 / PD-1 is a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]
Function CD279 / PD-1 is an inhibitory receptor on antigen activated T-cells. It plays a critical role in induction and maintenance of immune tolerance to self (PubMed:21276005). Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta.

The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28951311). The interaction with CD274/PDCD1L1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:28951311). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:22658127, PubMed:25034862, PubMed:25399552). [UniProt]
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Calculated MW 32 kDa