ARG65354

anti-CD28 antibody [CD28.2] (low endotoxin)

anti-CD28 antibody [CD28.2] (low endotoxin) for Flow cytometry,Functional study,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Primates

Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [CD28.2] recognizes CD28
Tested Reactivity Hu, NHuPrm
Tested Application FACS, FuncSt, ICC/IF, IHC-Fr, IP, WB
Specificity The clone CD28.2 reacts with CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.
HLDA V.; WS Code 5T CD28.05
Host Mouse
Clonality Monoclonal
Clone CD28.2
Isotype IgG1
Target Name CD28
Antigen Species Human
Immunogen DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
Conjugation Un-conjugated
Alternate Names Tp44; TP44; T-cell-specific surface glycoprotein CD28; CD antigen CD28

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 12 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note Functional studies: T cell costimulation.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Purification Note 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 940 Human CD28

Swiss-port # P10747 Human T-cell-specific surface glycoprotein CD28

Gene Symbol CD28
Gene Full Name CD28 molecule
Background CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.
Function Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. [UniProt]
Research Area Developmental Biology antibody; Immune System antibody; Cytotoxic T Cell Surface Study antibody
Calculated MW 25 kDa
PTM CD40LG induces tyrosine phosphorylation of isoform 3.

Clone References

Antipsychotic drugs suppress the AKT/NF-κB pathway and regulate the differentiation of T-cell subsets.

FuncSt / Human

Chen ML et al.
Immunol Lett.,  (2011)

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NFATc3 regulates the transcription of genes involved in T-cell activation and angiogenesis.

Urso K et al.
Blood.,  (2011)

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Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates.

Jeong SH et al.
J Virol.,  (2004)

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Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling.

Scharschmidt E et al.
Mol Cell Biol.,  (2004)

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