ARG54690
anti-CD284 / TLR4 antibody
anti-CD284 / TLR4 antibody for ELISA,Western blot,ICC/IF and Human
Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes CD284 / TLR4 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | CD284 / TLR4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide (15 aa) within aa. 30-80 of Human TLR4. |
| Conjugation | Un-conjugated |
| Alternate Names | CD284; CD antigen CD284; ARMD10; hToll; TLR-4; TOLL; Toll-like receptor 4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | PC-3 Cell Lysate |
Properties
| Form | Liquid |
|---|---|
| Purification | Ion exchange chromatography purified. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TLR4 |
| Gene Full Name | toll-like receptor 4 |
| Background | TLR4 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis. |
| Function | Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. [UniProt] |
| Highlight | Related products: TLR4 antibodies; TLR4 ELISA Kits; Anti-Rabbit IgG secondary antibodies; Related news: Detecting exosomal HMGB1 for ICD research |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody |
| Calculated MW | 96 kDa |
| PTM | N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS. |
