ARG54690

anti-CD284 / TLR4 antibody

anti-CD284 / TLR4 antibody for ELISA,Western blot,ICC/IF and Human

Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CD284 / TLR4
Tested Reactivity Hu
Tested Application ELISA, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CD284 / TLR4
Antigen Species Human
Immunogen Synthetic peptide (15 aa) within aa. 30-80 of Human TLR4.
Conjugation Un-conjugated
Alternate Names CD284; CD antigen CD284; ARMD10; hToll; TLR-4; TOLL; Toll-like receptor 4

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF2 μg/ml
WB2.5 - 5 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control PC-3 Cell Lysate

Properties

Form Liquid
Purification Ion exchange chromatography purified.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7099 Human TLR4

Swiss-port # O00206 Human Toll-like receptor 4

Gene Symbol TLR4
Gene Full Name toll-like receptor 4
Background TLR4 Antibody: Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-κB. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.
Function Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species-specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. [UniProt]
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TLR4 antibodies; TLR4 ELISA Kits; Anti-Rabbit IgG secondary antibodies;
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Research Area Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW 96 kDa
PTM N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.