ARG54702

Name: anti-CD284 / TLR4 antibody
Brand: arigo Biolaboratories
SKU: ARG54702
Price: 345 USD
Availability: InStock
Rating: 5.0 (2 reviews)

anti-CD284 / TLR4 antibody

Publication5

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Package

Price (USD)

100 μl

345.00

50 μl

245.00

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Overview

Product Description	Rabbit Polyclonal antibody recognizes CD284 / TLR4
Tested Reactivity	Hu, Ms, Rat
Predict Reactivity	Bov, Hm, Pig
Tested Application	FACS, WB
Host	Rabbit
Clonality	Polyclonal
Target Name	CD284 / TLR4
Antigen Species	Human
Immunogen	KLH-conjugated synthetic peptide corresponding to aa. 669-698 (Center) of Human TLR4 (NP_003257.1).
Conjugation	Un-conjugated
Alternate Names	CD284; CD antigen CD284; ARMD10; hToll; TLR-4; TOLL; Toll-like receptor 4

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	1:10 - 1:50
WB	1:1000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification	Protein A purified
Buffer	PBS and 0.09% (W/V) Sodium azide
Preservative	0.09% (W/V) Sodium azide
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 21898 Mouse TLR4 GeneID: 29260 Rat TLR4 GeneID: 7099 Human TLR4 Swiss-port # O00206 Human Toll-like receptor 4 Swiss-port # Q9QUK6 Mouse Toll-like receptor 4 Swiss-port # Q9QX05 Rat Toll-like receptor 4 More
Gene Symbol	TLR4
Gene Full Name	toll-like receptor 4
Background	The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Function	Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by free fatty acids, such as palmitate, and Ni(2+). Responses triggered by Ni(2+) require non-conserved histidines and are, therefore, species- specific. In complex with TLR6, promotes sterile inflammation in monocytes/macrophages in response to oxidized low-density lipoprotein (oxLDL) or amyloid-beta 42. In this context, the initial signal is provided by oxLDL- or amyloid-beta 42-binding to CD36. This event induces the formation of a heterodimer of TLR4 and TLR6, which is rapidly internalized and triggers inflammatory response, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. [From Uniprot]
Cellular Localization	Cell membrane; Single-pass type I membrane protein. Note=Upon complex formation with CD36 and TLR6, internalized through dynamin-dependent endocytosis
Highlight	Related products: TLR4 antibodies; TLR4 ELISA Kits; Related news: Detecting exosomal HMGB1 for ICD research
Research Area	Cell Biology and Cellular Response antibody; Immune System antibody; Microbiology and Infectious Disease antibody
Calculated MW	96 kDa
PTM	N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.