ARG22982

anti-CD29 / Integrin beta 1 antibody [12G10]

anti-CD29 / Integrin beta 1 antibody [12G10] for Electron microscopy,ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [12G10] recognizes CD29 / Integrin beta 1
Mouse anti Human CD29 monoclonal antibody, clone 12G10 recognizes human CD29 also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130 kDa under reducing, ~115 kDa under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin , collagen and fibronectin. the VLA heterodimers ediate cell-cell and cell-matrix interactions.Mouse anti human CD29, clone 12G10 binding to cells adhering via VLA-4 results in actin cytoskeletal disruption and subsequent inhibition of attachment and spreading whilst 12G10 binding to cells adhering via VLA-5 results in enhancement of both these processes (Humphries et al. 2005). Clone 12G10 enhances alpha 5 beta 1-fibronectin interactions and binds to a region of CD25 containing the binding epitopes of several other anti CD29 antibody clones. However, unlike these, binding of 12G10 is enhanced in the presence of ligands such as fibronectin fragments (Mould et al. 1995). Binding of antibody clone 12G10 to the integrin β1 subunit is affected by divalent cations and the binding epitiope appears to be located around residues 207-218 in the b1 subunit putative A-domain (Mould et al. 1998)
Tested Reactivity Hu
Species Does Not React With Ms, Rat
Tested Application ELISA, EM, FACS, ICC/IF, IHC-Fr, IP, WB
Specificity The clone 12G10 binds to the CD29 / Integrin beta 1 βI domain then activates and stabilize the open headpiece conformation (active CD29 / Integrin beta 1).
Host Mouse
Clonality Monoclonal
Clone 12G10
Isotype IgG1
Target Name CD29 / Integrin beta 1
Antigen Species Human
Immunogen Purified Human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.
Conjugation Un-conjugated
Alternate Names CD29; Glycoprotein IIa; Fibronectin receptor subunit beta; VLAB; MSK12; CD antigen CD29; FNRB; GPIIA; VLA-4 subunit beta; VLA-BETA; MDF2; Integrin beta-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA10 µg/ml
EMAssay-dependent
FACS1:25 - 1:50
ICC/IF1:25-1:500
IHC-Fr1:25-1:500
IP1:25-1:500
WB1:100-1:2000 (under non-reduced conditions only)
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3688 Human ITGB1

Swiss-port # P05556 Human Integrin beta-1

Gene Symbol ITGB1
Gene Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Background Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion.

Isoform 5: Isoform 5 displaces isoform 1 in striated muscles. [UniProt]
Highlight Related products:
CD29 antibodies; Anti-Mouse IgG secondary antibodies;
Calculated MW 88 kDa
PTM The cysteine residues are involved in intrachain disulfide bonds.