ARG59346

anti-CD2AP antibody [5F8]

anti-CD2AP antibody [5F8] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot,Immunocytochemistry and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [5F8] recognizes CD2AP
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 5F8
Isotype IgG1
Target Name CD2AP
Antigen Species Human
Immunogen Recombinant protein corresponding to K253-K337 of Human CD2AP.
Conjugation Un-conjugated
Alternate Names Adapter protein CMS; CD2-associated protein; CMS; Cas ligand with multiple SH3 domains

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 3 µg/10^6 cells
ICC1:200 - 1:1000
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose.
Preservative 0.05% Sodium azide
Stabilizer 4% Trehalose
Concentration 0.5 - 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12488 Mouse CD2AP

GeneID: 23607 Human CD2AP

Swiss-port # Q9JLQ0 Mouse CD2-associated protein

Swiss-port # Q9Y5K6 Human CD2-associated protein

Gene Symbol CD2AP
Gene Full Name CD2-associated protein
Background This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq, Jul 2008]
Function Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. In collaboration with CBLC, modulates the rate of RET turnover and may act as regulatory checkpoint that limits the potency of GDNF on neuronal survival. Controls CBLC function, converting it from an inhibitor to a promoter of RET degradation. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Also required for cytokinesis. [UniProt]
Cellular Localization Cytoplasm, cytoskeleton. Cell projection, ruffle. Cell junction. Note=Colocalizes with F-actin and BCAR1/p130Cas in membrane ruffles (PubMed:10339567). Located at podocyte slit diaphragm between podocyte foot processes (By similarity). During late anaphase and telophase, concentrates in the vicinity of the midzone microtubules and in the midbody in late telophase (PubMed:15800069). [UniProt]
Calculated MW 71 kDa
PTM Phosphorylated on tyrosine residues; probably by c-Abl, Fyn and c-Src. [UniProt]