ARG62807
anti-CD3 (activation epitope) antibody [APA1/1]
anti-CD3 (activation epitope) antibody [APA1/1] for Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse
Cancer antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [APA1/1] recognizes CD3 (activation epitope) |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | FACS, ICC/IF, IHC-Fr, IP, WB |
| Specificity | The clone APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | APA1/1 |
| Isotype | IgG1 |
| Target Name | CD3 (activation epitope) |
| Antigen Species | Human |
| Immunogen | Purified human CD3 proteins isolated from thymus. |
| Conjugation | Un-conjugated |
| Alternate Names | T-cell receptor T3 delta chain; T3D; IMD19; CD3-DELTA; CD antigen CD3d; T-cell surface glycoprotein CD3 delta chain |
Application Instructions
| Application Suggestion |
|
||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | FACS: Sample preparation: At the end of stimulation of T cells, perform staining of surface markers (if required) in PBS + 0.1% BSA for 20 min on ice. Wash with PBS and fix with 2% formaldehyde, 30 min on ice. Wash with PBS and incubate in PBS + 0.1% saponine, 5 min at RT. Incubate the cells in PBS + 1% BSA + 0.03% saponine, 15 min on ice. Incubate with fluorescence-labeled APA1/1 antibody (1-2 μg/ml) in PBS + 1% BSA + 0.03% saponine in dark, 20 min at RT. Wash with PBS + 1% BSA + 0.03% saponine, resuspend in PBS. ICC/IF: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||||
| Positive Control | FACS: Human T cells stimulated with ARG62802 anti-CD3 antibody [MEM-57] at 1 µg/ml dilution. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from cell culture supernatant by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04234 Human T-cell surface glycoprotein CD3 delta chain Swiss-port # P04235 Mouse T-cell surface glycoprotein CD3 delta chain |
|---|---|
| Gene Symbol | CD3D |
| Gene Full Name | CD3d molecule, delta (CD3-TCR complex) |
| Background | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. |
| Function | The CD3 complex mediates signal transduction. [UniProt] |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 19 kDa |
