ARG53823

anti-CD3 (activation epitope) antibody [APA1/1] (PE)

anti-CD3 (activation epitope) antibody [APA1/1] (PE) for Flow cytometry and Human,Mouse

Cancer antibody; Developmental Biology antibody; Immune System antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [APA1/1] recognizes CD3 (activation epitope)
Tested Reactivity Hu, Ms
Tested Application FACS
Specificity The clone APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
Host Mouse
Clonality Monoclonal
Clone APA1/1
Isotype IgG1
Target Name CD3 (activation epitope)
Antigen Species Human
Immunogen Purified human CD3 proteins isolated from thymus.
Conjugation PE
Alternate Names T-cell receptor T3 delta chain; T3D; IMD19; CD3-DELTA; CD antigen CD3d; T-cell surface glycoprotein CD3 delta chain

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12500 Mouse CD3D

GeneID: 915 Human CD3D

Swiss-port # P04234 Human T-cell surface glycoprotein CD3 delta chain

Swiss-port # P04235 Mouse T-cell surface glycoprotein CD3 delta chain

Gene Symbol CD3D
Gene Full Name CD3d molecule, delta (CD3-TCR complex)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Function The CD3 complex mediates signal transduction. [UniProt]
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Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 19 kDa