ARG23315
anti-CD3 antibody [1F4]
anti-CD3 antibody [1F4] for IHC-Frozen sections,Flow cytometry,Immunoprecipitation,IHC-Formalin-fixed paraffin-embedded sections and Rat
Overview
| Product Description | Mouse Monoclonal antibody [1F4] recognizes CD3 CD3 antibody, clone 1F4 recognizes rat CD3 found on rat T-cells. It stains rat thymus tissues strongly in the medulla and weakly in the cortex and induces the proliferation of T-cells in the presence of PMA. |
|---|---|
| Tested Reactivity | Rat |
| Tested Application | FACS, IHC-Fr, IHC-P, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1F4 |
| Isotype | IgM |
| Target Name | CD3 |
| Antigen Species | Rat |
| Immunogen | F344 Rat T cells stimulated with PMA (TPA) and calcium ionophore. |
| Conjugation | Un-conjugated |
| Alternate Names | CD3-GAMMA; T3G; T-cell receptor T3 gamma chain; CD antigen CD3g; T-cell surface glycoprotein CD3 gamma chain; IMD17 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: This clone is suitable for use on paraffin-embedded material using target unmasking fluid. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by ammonium sulfate precipitation. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q64159 Rat T-cell surface glycoprotein CD3 gamma chain |
|---|---|
| Gene Symbol | CD3G |
| Gene Full Name | CD3g molecule, gamma (CD3-TCR complex) |
| Background | The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008] |
| Function | The CD3 complex mediates signal transduction. [UniProt] |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Mouse IgM secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| Calculated MW | 20 kDa |
