ARG23049
anti-CD3 antibody [KT3]
anti-CD3 antibody [KT3] for IHC-Frozen sections,Flow cytometry and Mouse
Overview
| Product Description | Rat Monoclonal antibody [KT3] recognizes CD3 CD3 antibody, clone KT3 recognizes the mouse CD3 antigen expressed by mature T cells. Clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, IHC-Fr |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | KT3 |
| Isotype | IgG2a |
| Target Name | CD3 |
| Antigen Species | Mouse |
| Immunogen | CBAT6 thymocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | CD3-GAMMA; T3G; T-cell receptor T3 gamma chain; CD antigen CD3g; T-cell surface glycoprotein CD3 gamma chain; IMD17 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | IHC-Fr: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11942 Mouse T-cell surface glycoprotein CD3 gamma chain |
|---|---|
| Gene Symbol | Cd3g |
| Gene Full Name | CD3 antigen, gamma polypeptide |
| Background | The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008] |
| Function | The CD3 complex mediates signal transduction. [UniProt] |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Rat IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| Calculated MW | 20 kDa |
