ARG65499
anti-CD3 antibody [MEM-57] (low endotoxin)
anti-CD3 antibody [MEM-57] (low endotoxin) for CyTOF®-candidate,Flow cytometry,Functional study,Immunoprecipitation and Human
Cancer antibody; Developmental Biology antibody; Immune System antibody
Overview
| Product Description | Azide free and low endotoxin Mouse Monoclonal antibody [MEM-57] recognizes CD3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | CyTOF®-candidate, FACS, FuncSt, IP |
| Specificity | The clone MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. HLDA IV.; WS Code T 96 |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | MEM-57 |
| Isotype | IgG2a |
| Target Name | CD3 |
| Antigen Species | Human |
| Immunogen | Human thymocytes and T lymphocytes. |
| Conjugation | Un-conjugated |
| Alternate Names | T-cell receptor T3 delta chain; T3D; IMD19; CD3-DELTA; CD antigen CD3d; T-cell surface glycoprotein CD3 delta chain |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IP: The clone MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28 kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7). Functional studies: The clone MEM-57 has strong mitogenic effect on peripheral T lymphocytes; It reacts strongly with gamma/delta T lymphocytes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||||||
| Positive Control | FACS: Jurkat |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Purification Note | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P04234 Human T-cell surface glycoprotein CD3 delta chain |
|---|---|
| Gene Symbol | CD3D |
| Gene Full Name | CD3d molecule, delta (CD3-TCR complex) |
| Background | CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. |
| Function | The CD3 complex mediates signal transduction. [UniProt] |
| Highlight | Related products: CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: New antibody panels and duos for Tumor immune microenvironment Tumor-Infiltrating Lymphocytes (TILs) Exploring Antiviral Immune Response |
| Research Area | Cancer antibody; Developmental Biology antibody; Immune System antibody |
| Calculated MW | 19 kDa |
Clone References
Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells.
FACS /
Lck mediates signal transmission from CD59 to the TCR/CD3 pathway in Jurkat T cells.
Pfit is a structurally novel Crohn's disease-associated superantigen.
A selective inhibitor reveals PI3Kγ dependence of T(H)17 cell differentiation.
A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.
FACS / Human
